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Microarray Analysis of Gene Expression in Huntington's Disease Peripheral Blood - a Platform Comparison

Microrray Platform: Affymetrix® GeneChip® Human Genome U133A and CodeLink Human UniSet 20K

Reference:
Affymetrix data available from Gene Expression Omnibus (GEO), accession number GSE1751, and CodeLink data in GEO dataset GSE1767. Originally reported in Boroveki, et al 2005.

Abstract:
Gene expression in human blood from symptomatic HD patients was compared to that in blood from healthy donors using both the Affymetrix GeneChip Human Genome U133A and the CodeLink Human UniSet 20K microarrays. Microarray analysis demonstrates that this neurodegenerative disorder results in significant differential gene expression in peripheral blood. Comparison of the two datasets revealed a high degree of agreement between the two platforms, both at the individual gene level (~65% overlap) and in the gene ontologies identified as being enriched in each dataset. Biological processes identified as significantly over-represented among differentially expressed genes by both platforms include mRNA splicing, the ubiquitin cycle and apoptosis.

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